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By J. C. Schoute

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2 (hyperglycosylated hCG) [19]. The acidity repels circulating hCG from binding the renal glomerular basement membrane. 43-h circulating half-life of its nonacidic sister hormone, leutinizing hormone (LH) [20]. hCG completely lacks the aromatic amino acid tryptophan. 41 3 104 M21 cm21 [21]). The sequence of the C-terminal extension on the β-subunit of hCG (the site of the four O-linked oligosaccharides) is like a proteoglycan sequence, a polymer of Ser and Pro. The C-terminal sequence formed between residues 118 and 145 consists of 27 amino acids, including 8 Ser and 8 Pro residues.

2) [5,6]. 2). Recent mass spectrometry studies show that all hCG molecules contain one hexasaccharide/ pentasaccharide structure at residue 121, regardless of the source of hCG [9]. 2 O-linked oligosaccharides on hCG and hyperglycosylated hCG. (A) Simple and (B) complex. However, if this were true, then the average pregnancy hCG would contain 25% hexasaccharide/pentasaccharide, rather than the 15% indicated by glycosidase and specialized HPLC studies [6]. 3) [6]. 3). 9% triantennary and the β-subunit contains 16% triantennary oligosaccharides.

45] Otani T, Boime I, Mochizuki M. Studies on the transcriptional regulatory elements of the hCG beta gene cluster using a choriocarcinoma cell line which expresses hCG beta eutopically. Nippon Sanka Fujinka Gakkai Zasshi 1989;41:1885À90. [46] Hollenberg AN, Pestell RG, Albanese C, Boers ME, Jameson JL. Multiple promoter elements in the human chorionic gonadotropin beta subunit genes distinguish their expression from the luteinizing hormone beta gene. Mol Cell Endocrinol 1994;106: 111À19. [47] Albanese C, Kay TW, Troccoli NM, Jameson JL.

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